Additionally, intrarenal oxidative stress was assessed by the indicators malondialdehyde, 8-isoprostane, oxidized-low density lipoprotein and 8-hydroxy-2вЂ™-deoxyguanosine, as well as the activity levels of the antioxidant enzymes total superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and Оі-glutamylcysteine synthetase. After 24 weeks, renal histopathology was evaluated according to the Banff criteria after hematoxylin and eosin, MassonвЂ™s trichrome and periodic acid-Schiff stainings. Renal function and 24-hour urinary protein were monitored for variations for 24 weeks after transplantation.

Sulforaphane was administered at 1.5 mg/kg intraperitoneally once daily. Methods: F344 rat kidneys were orthotopically transplanted into Lewis rat recipients to establish CRAD models.

The present paper investigates whether there is an association between oxidative stress alleviation via sulforaphane-induced Nrf2-HO-1/NQO-1 signaling pathway activation and CRAD improvement. An antioxidant intervention via Nrf2 signaling pathway activation might be a promising therapy for some kidney diseases. Oxidative stress may account for the nonspecific interstitial fibrosis and tubular atrophy that occur in CRAD. Background/Aims: Chronic renal allograft dysfunction (CRAD) is a leading cause of long-term renal allograft loss.

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